Jonathan and James Davies will become the first set of brothers since 2006 to start for Wales when they feature against England on Saturday.
Wales head coach Warren Gatland has named Scarlets openside flanker James alongside back-row colleagues Aaron Wainwright and Ross Moriarty for the World Cup warm-up clash in Cardiff.
Jonathan, meanwhile, partners Hadleigh Parkes in midfield as Wales look to bounce back from a 33-19 loss to England at Twickenham last weekend.
They are the first set of brothers to play alongside each other for Wales since Jamie and Nicky Robinson 13 years ago.
James Davies replaces Justin Tipuric, while two other changes from the England defeat see fly-half Dan Biggar in for an injured Gareth Anscombe and lock Jake Ball replacing Adam Beard.
Biggar starts with Anscombe having suffered a World Cup-ending knee ligament injury at Twickenham.
And Cardiff Blues forward Josh Navidi is among the replacements, along with his Blues colleague and fly-half Jarrod Evans.
Gatland said: "This weekend is another opportunity for us to take the field, test ourselves and continue our preparation for Japan.
"It is a little over five weeks until we kick off our campaign in Toyota against Georgia, and it is important we are fully prepared and rugby-ready by then.
"We were disappointed to get the loss last weekend, so it is important we step it up on Saturday and put in a big display in front a of a packed Principality Stadium.
"The squad are looking forward to getting back out there, taking another step towards the World Cup and putting in a big performance."
Wales are unbeaten at the Principality Stadium since world champions New Zealand toppled them in November 2017, since when they have reeled off 10 successive victories.
— Welsh Rugby Union (@WelshRugbyUnion) August 15, 2019
Wales team to play England
Forwards: 1 Nicky Smith (Ospreys), 2 Ken Owens (Scarlets), 3 Tomas Francis (Exeter Chiefs), 4 Jake Ball (Scarlets), 5 Alun Wyn Jones (CAPTAIN, Ospreys), 6 Aaron Wainwright (Dragons), 7 James Davies (Scarlets), 8 Ross Moriarty (Dragons)
Backs: 9 Gareth Davies (Scarlets), 10 Dan Biggar (Northampton Saints), 11 Josh Adams (Cardiff Blues), 12 Hadleigh Parkes (Scarlets), 13 Jonathan Davies (Scarlets), 14 George North (Ospreys), 15 Liam Williams (Saracens)
Replacements: 16 Elliot Dee (Dragons), 17 Wyn Jones (Scarlets), 18 Dillon Lewis (Cardiff Blues), 19 Aaron Shingler (Scarlets), 20 Josh Navidi (Cardiff Blues), 21 Aled Davies (Ospreys), 22 Jarrod Evans (Cardiff Blues), 23 Owen Watkin (Ospreys)